Why are fungal cultivation chambers used now?

The main checks performed using strains include the following aspects:

Strain purity and characteristic confirmation: Standard reserve strains should undergo purity and characteristic confirmation. Purity confirmation can be performed using the streak method, while characteristic confirmation can be performed using methods such as Gram staining and API identification.

Strain preservation and management: Strain preservation and management must comply with relevant laws and regulations, such as the "People's Republic of China Infectious Disease Prevention and Control Law" and the "Regulations on the Biosafety Management of Pathogenic Microorganism Laboratories". Strains should be managed by a dedicated person, using a two-person management system, and must not be retained without authorization. Strain files should include information such as source, name, number, preservation conditions, and custodian.

Strain quality testing methods: Commonly used quality testing methods include establishing standard cultivation and observation methods, continuous observation, and macroscopic inspection. By comparing the biological characteristics, morphological characteristics, and physiological and ecological characteristics of the strains, it is possible to detect whether the strains have problems such as degeneration, aging, or contamination.

Strain identification and detection items: Common strain identification and detection items include total bacterial count, coliform group, molds and yeasts, Staphylococcus aureus, Salmonella, Shigella, and Vibrio parahaemolyticus. These items can reflect the quantity and types of strains in a sample and are important for evaluating food quality and safety.

Detection standards and methods: The standards for strain identification and detection generally follow relevant national regulations and international standards. Detection methods include traditional culture methods, rapid detection methods, and molecular biology methods. Traditional culture methods are simple to operate but time-consuming; rapid detection methods are quick but may have errors; molecular biology methods are highly accurate but complex and costly to operate.